How to Build a Single-Cell RNA-seq Analysis Pipeline with Scanpy for PBMC Clustering, Annotation, and Trajectory Discovery
In this tutorial, we carry out a sophisticated single-cell RNA-seq evaluation workflow utilizing Scanpy on the PBMC-3k benchmark dataset. We begin by loading the dataset, inspecting its construction, and making use of high quality management checks to consider gene counts, complete counts, mitochondrial content material, and ribosomal gene alerts. We then filter low-quality cells and…
